Search results for "three-dimensional microscopy"

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Fast Inertia-Free Volumetric Light-Sheet Microscope

2017

Fast noninvasive three-dimensional (3D) imag-ing is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characterization of rapidly evolving systems. However, to obtain a 3D image, either the sample or parts of the microscope are moved, limiting the acquisition speed. Here, we propose a novel inertia-free light-sheet-based scheme for volumetric imaging at high temporal resolution. Our approach comprises a novel combination of an acousto-optic scanner to produce tailored illumination and an acoustic-optofluidic lens, placed in the detection path to provide extended dept…

0301 basic medicineScanneracouto-optic devicesMaterials scienceMicroscopethree-dimensional microscopy01 natural sciencesAcouto-optic devices flow cytometry light-sheet microscopy three-dimensional microscopy Electronic Optical and Magnetic Materials Biotechnology Atomic and Molecular Physics and Optics Electrical and Electronic Engineeringlaw.invention010309 optics03 medical and health sciencesOpticslawAtomic and Molecular Physics0103 physical sciencesMicroscopyElectronicOptical and Magnetic MaterialsElectrical and Electronic Engineeringbusiness.industryflow cytometryRangingFrame rateAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsCharacterization (materials science)Lens (optics)acouto-optic devices; flow cytometry; light-sheet microscopy; three-dimensional microscopy; Electronic Optical and Magnetic Materials; Biotechnology; Atomic and Molecular Physics and Optics; Electrical and Electronic Engineering030104 developmental biologyLight sheet fluorescence microscopyand Opticsbusinesslight-sheet microscopyBiotechnologyACS Photonics
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Large Depth-of-Field Integral Microscopy by Use of a Liquid Lens

2018

Integral microscopy is a 3D imaging technique that permits the recording of spatial and angular information of microscopic samples. From this information it is possible to calculate a collection of orthographic views with full parallax and to refocus computationally, at will, through the 3D specimen. An important drawback of integral microscopy, especially when dealing with thick samples, is the limited depth of field (DOF) of the perspective views. This imposes a significant limitation on the depth range of computationally refocused images. To overcome this problem, we propose here a new method that is based on the insertion, at the pupil plane of the microscope objective, of an electrical…

MicroscopeComputer scienceOptical powerthree-dimensional image acquisitionthree-dimensional microscopylcsh:Chemical technology01 natural sciencesBiochemistryPupilArticlethree-dimensional image processingAnalytical Chemistrylaw.invention010309 opticsOpticslaw0103 physical sciencesMicroscopylcsh:TP1-1185Depth of fieldElectrical and Electronic EngineeringInstrumentation010302 applied physicsbusiness.industryOrthographic projectionAtomic and Molecular Physics and OpticsNumerical apertureMicroscòpiaCardinal pointFocus (optics)ParallaxbusinessImatges ProcessamentSensors
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